Monkey HS-INS (High Sensitivity Insulin) Accquant® ELISA Kit AP-TNAP Only those wells that contain

Only those wells that contain Rat Aβ1-42

The concentration of Dog IL1a in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human TSP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Universal BH4 in the sample or standard competes with a fixed amount of Universal BH4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal BH4

Monkey HS-INS (High Sensitivity Insulin) Accquant® ELISA Kit AP-TNAP Only those wells that containThis High Sensitivity Insulin ELISA kit is based on Sandwich ELISA detection method and takes approximately 4 hours. The microplate provided in this kit has been precoated with anti INS antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with INS bound to precoated antibody. Wash unbound components and add HRP Streptavidin