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Then a biotinylated detection antibody specific for Rat UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Luciferase produces green light with the wavelength of 562 nm
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VASP
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LRP6
Human ADCY6 (Adenylate Cyclase Type 6) ELISA Kit Monocyte Differentiation Antigen CD14 Then a biotinylated detection antibodyThe Human (ADCY6) Adenylate Cyclase Type 6 ELISA Kit measures Adenylate Cyclase Type 6 in samples. The plate has been pre coated with Human ADCY6 antibody. ADCY6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ADCY6 Antibody is added and binds to ADCY6 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ADCY6 antibody. After incubation unbound Streptavidin HRP is washed
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