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When the sample containing p53 is added to the plate
The concentration of Human AFF1 in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Human FIS-1 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated EPN2 antibody
Sheep CLU (Clusterin) ELISA Kit Packaging:96 wells When the sample containing p53The Sheep (CLU) Clusterin ELISA Kit measures Clusterin in Sheep samples. The plate has been pre coated with Sheep CLU antibody. CLU present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep CLU Antibody is added and binds to CLU in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CLU antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
